Detection of Human Torovirus Like Particles and Adenovirus Type F in Children Attending to Babylon Maternity and Children Hospital

摘要: Toroviruses are enteric viruses belonging to the Nidovirales order that infect different animal species and human . Torovirus-like particales (TVLPs) immunologically related BRV have been reported as etiological agents of gastroenteritis in humans. The lack “in vitro” culture systems for toroviruses, except  prototype Berne virus or BEV, isolated originally from an infected horse, has hampered their study development diagnostic assays. This  study  describes a real time RT-PCR method detect  human  torovirus- like particles  RNA clinical  stool  samples using primers corresponding gene coding for  nucleocapsid protein which conserved all strains known date. During this study, CT value  measured  during real-time PCR analysis was used indication viral load found sample assay evaluated with 72 stool children attending Babylon maternity hospital. Fifty tow out (72.2%) were shedding at  collection, indicating high incidence TVLPs  infection  Province. is first attempted  for   estimating  presence Iraq. described provides rapid, highly sensitive, specific reliable detection quantization  enabling future epidemiological studies. In addition that  included assays group F Adenovirus 250 pediatric subjects  exhibiting symptoms diarrhea and/ vomiting  which  examined. results 10 positive diarrheic confirmed by electron microscopy  examination gave clear appearance Till now there no successful virus  growth isolation diarrhegenic type 40 41 grow routine cell result reverse transcription  – PCR  reflected .2 % 58 torovirus adenovirus respectively. genotyping adenoviruses(genotype 41)  highlight significance rapid molecular methods screening laboratories provide efficient Keywords : Human Torovirus, Adenovirus, RT-PCR, Electron Microscopy.