Histone H1: an antimicrobial protein of Atlantic salmon (Salmo salar).
作者： Robert C Richards , David B O'Neil , Pierre Thibault , K.Vanya Ewart
关键词: Antimicrobial 、 Peptide mass fingerprinting 、 Biochemistry 、 High-performance liquid chromatography 、 Molecular mass 、 Ammonium sulfate precipitation 、 Salmo 、 Chemistry 、 Antimicrobial peptides 、 Histone H1
摘要: Antimicrobial activity was detected in acid extracts of liver, intestine, and stomach healthy Atlantic salmon (Salmo salar). An antimicrobial protein isolated from liver using extraction followed by ammonium sulfate precipitation, large-scale gel filtration chromatography, reverse-phase HPLC, size exclusion HPLC. The (SAM) found to have a molecular mass 20,734 Da MALDI TOF spectrometry. Peptide fingerprinting partial sequencing tandem nanoelectrospray spectrometry identified the as histone H1. had minimal inhibitory concentration 31 μg/mL against E. coli D31 plate clearing assay. effect SAM on bacterial morphology indistinguishable that (Ala-8,13,18)-magainin II, shown scanning electron microscopy, which suggests disrupts membranes manner similar most peptides. This may act an vivo through active secretion or release cells during infection-related apoptosis.
elsevier.com
sci-hub.se 参考文章(28)
S. J. BOLTON, V. H. PERRY, Histone H1; a neuronal protein that binds bacterial lipopolysaccharide. Journal of Neurocytology. ,vol. 26, pp. 823- 831 ,(1997) , 10.1023/A:1018574600961
Ultrastructure techniques for microorganisms Plenum Press. ,(1986) , 10.1007/978-1-4684-5119-1
P. Roepstorff, J. Fohlman, Letter to the editors Biological Mass Spectrometry. ,vol. 11, pp. 601- 601 ,(1984) , 10.1002/BMS.1200111109
P S Hiemstra, P B Eisenhauer, S S Harwig, M T van den Barselaar, R van Furth, R I Lehrer, Antimicrobial proteins of murine macrophages. Infection and Immunity. ,vol. 61, pp. 3038- 3046 ,(1993) , 10.1128/IAI.61.7.3038-3046.1993
A Zychlinsky, P Sansonetti, Perspectives series: host/pathogen interactions. Apoptosis in bacterial pathogenesis. Journal of Clinical Investigation. ,vol. 100, pp. 493- 495 ,(1997) , 10.1172/JCI119557
D. Robinette, S. Wada, T. Arroll, M. G. Levy, W. L. Miller, E. J. Noga, Antimicrobial activity in the skin of the channel catfish Ictalurus punctatus: characterization of broad-spectrum histone-like antimicrobial proteins Cellular and Molecular Life Sciences. ,vol. 54, pp. 467- 475 ,(1998) , 10.1007/S000180050175
K Brix, W Summa, F Lottspeich, V Herzog, Extracellularly occurring histone H1 mediates the binding of thyroglobulin to the cell surface of mouse macrophages. Journal of Clinical Investigation. ,vol. 102, pp. 283- 293 ,(1998) , 10.1172/JCI1614
Alexander M. Cole, Peddrick Weis, Gill Diamond, Isolation and Characterization of Pleurocidin, an Antimicrobial Peptide in the Skin Secretions of Winter Flounder Journal of Biological Chemistry. ,vol. 272, pp. 12008- 12013 ,(1997) , 10.1074/JBC.272.18.12008
Richard M. Epand, Hans J. Vogel, Diversity of antimicrobial peptides and their mechanisms of action. Biochimica et Biophysica Acta. ,vol. 1462, pp. 11- 28 ,(1999) , 10.1016/S0005-2736(99)00198-4
Hao-Chia Chen, Judith H. Brown, John L. Morell, C.M. Huang, Synthetic magainin analogues with improved antimicrobial activity FEBS Letters. ,vol. 236, pp. 462- 466 ,(1988) , 10.1016/0014-5793(88)80077-2