Pannexin 1 regulates spiny protrusion dynamics in cortical neurons

摘要: The integration of neurons into networks relies on the formation dendritic spines. These specialized structures arise from dynamic filopodia-like spiny protrusions. Recently, it was discovered that cortical lacking channel protein Pannexin 1 (Panx1) exhibited larger and more complicated neuronal networks, as well as, higher spine densities. Here, we expanded those findings to investigate whether increase in density associated with lack Panx1 due differences dynamics. Using a fluorescent membrane tag (mCherryCD9-10) visualize protrusions developing (at 10 days-in-vitro, DIV10) confirmed leads protrusion while transient transfection decreased density. To quantify impact expression formation, elimination, lability motility, used live cell imaging DIV10 (1 frame every 5 seconds for minutes). We discovered, at DIV10, stabilized neuron Furthermore, knockout resulted significant turnover overall movement. In summary, these new data reveal regulates development spines by controlling