DNA methylation level of cyp19a1a and Foxl2 gene related to their expression patterns and reproduction traits during ovary development stages of Japanese flounder (Paralichthys olivaceus).
作者： Yufeng Si , Yuxia Ding , Feng He , Haishen Wen , Jifang Li
DOI: 10.1016/J.GENE.2015.09.006
关键词: Genetics 、 DNA methylation 、 Regulation of gene expression 、 Molecular biology 、 Gene 、 Gene expression 、 Biology 、 Methylation 、 CpG site 、 Bisulfite sequencing 、 FOXL2 Gene
摘要: Foxl2 and cyp19a1a genes are crucial for the ovarian development, could play a direct regulatory role on transcription. In this study, we aimed to study DNA methylation status mRNA expression patterns of during development female Japanese flounder. The relative level gene gonadal stages was measured by quantitative PCR. Moreover, in promoter coding regions two detected bisulfite sequencing. estradiol-17β (E2) radioimmunoassay. results showed low levels II V, while highest were stage IV. variation trend all CpG sites exon 1 three rich region negatively associated with their development. addition, seven putative transcription factors binding sequence. Further studies that forkhead domain, which is important located F1 F2 region. These provide powerful theoretical basis mechanism regulating promoting differentiation towards pathway, further reveal vital flounder gonad
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