Electrochemical detection of phenolic compounds using composite film of multiwall carbon nanotube/surfactant/tyrosinase on a carbon paste electrode
作者： S. Hashemnia , Sh. Khayatzadeh , M. Hashemnia
DOI: 10.1007/S10008-011-1355-2
关键词: Immobilized enzyme 、 Enzyme electrode 、 Carbon nanotube 、 Biosensor 、 Tyrosinase 、 Catechol 、 Phenol 、 Chemistry 、 Organic chemistry 、 Carbon paste electrode 、 Nuclear chemistry
摘要: A new tyrosinase-based biosensor was developed for detection of phenolic compounds using composite film multiwall carbon nanotube (MWCNT)/dimethylditetradecylammonium bromide (DTDAB)/tyrosinase (Tyr) on a Nafion-incorporated paste electrode. The showed sensitive electrochemical response to the reduction oxidation products different (phenol, catechol, p-cresol, and p-chlorophenol) by dissolved O2 in presence immobilized enzyme. effects pH, operating potential, MWCNT concentration, DTDAB/Tyr ratio were explored optimum analytical performance. exhibited linear range 1.5–25.0, 2.0–15.0, 2.5–25.0 μM sensitivity 2,900, 3,100, 1,500 μA/mM phenol, p-chlorophenol, respectively. In addition, enzyme electrode Michaelis–Menten behavior at concentrations higher than 5.0 μM. stability application also evaluated.
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