Relationship between copper(II) complexes with FomA adhesin fragments of F. nucleatum and colorectal cancer. Coordination pattern and ability to promote ROS production

摘要: The copper(II) ion binding of the Ac-KGHGNG-NH2 and Ac-PTVHNE-NH2 fragments FomA adhesin from Fusobacterium nucleatum was studied using potentiometry, UV-Vis, CD, EPR DFT techniques. coordination pattern described in a wide range pH values. Ligands begin interactions with metal ions imidazole nitrogen. At 6.8 (a value typical large intestine environment), coordinated by 3N donor atoms {Nim, 2 × N−amide} both cases. However, bound more effectively peptide. formation reactive oxygen species (ROS) UV-Vis fluorescence spectroscopy, as well gel electrophoresis presence H2O2 and/or ascorbic acid. complexes generated ROS highest amounts among all compounds. Moreover, they stimulated CT26 cell line (mouse colon carcinoma) to produce which lead oxidative stress. It also determined that such radicals took part plasmid degradation mechanism.