Amino acid substitutions in the structural or nonstructural proteins of a vaccine strain of equine arteritis virus are associated with its attenuation
作者： Jianqiang Zhang , Yun Young Go , N. James MacLachlan , Barry J. Meade , Peter J. Timoney
DOI: 10.1016/J.VIROL.2008.06.003
关键词: Reverse genetics 、 Amino acid 、 Strain (chemistry) 、 Virology 、 Biology 、 Recombinant DNA 、 Virulence 、 RNA-dependent RNA polymerase 、 Recombinant virus 、 Attenuated vaccine
摘要: Comparative sequence analysis of a series strains equine arteritis virus (EAV) defined virulence for horses, ranging from the horse-adapted virulent Bucyrus (VB) strain to fully attenuated vaccine derived it, identified 13 amino acid substitutions associated with attenuation. These include 4 in replicase proteins and 9 structural proteins. Using reverse genetic techniques, these were introduced into infectious cDNA clone pEAVrVBS VB EAV. Inoculation horses recombinant viruses clearly demonstrated that changes either (nsp1, nsp2 nsp7) or (GP2, GP4, GP5 M) resulted attenuation strain. The was more than only
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Mccollum Wh, Bryans Jt, Crowe Me, Doll Er, Isolation of a filterable agent causing arteritis of horses and abortion by mares; its differentiation from the equine abortion (influenza) virus. The Cornell veterinarian. ,vol. 47, pp. 3- 41 ,(1957)
Cavanagh D, Nidovirales: a new order comprising Coronaviridae and Arteriviridae. Archives of Virology. ,vol. 142, pp. 629- 633 ,(1997)
E J Snijder, J J Meulenberg, The molecular biology of arteriviruses Journal of General Virology. ,vol. 79, pp. 961- 979 ,(1998) , 10.1099/0022-1317-79-5-961
Moraillon R, Moraillon A, Results of an epidemiological investigation on viral arteritis in France and some other European and African countries. Annals of veterinary research. ,vol. 9, pp. 43- 54 ,(1978)
Peter J. Timoney, William H. McCollum, Equine viral arteritis. Veterinary Clinics of North America-equine Practice. ,vol. 9, pp. 295- 309 ,(1993) , 10.1016/S0749-0739(17)30397-8
D. W. C. Beasley, C. T. Davis, M. Whiteman, B. Granwehr, R. M. Kinney, Allan D. T. Barrett, Molecular determinants of virulence of West Nile virus in North America. Archives of virology. Supplementum. pp. 35- 41 ,(2004) , 10.1007/978-3-7091-0572-6_4
R M Kinney, G J Chang, K R Tsuchiya, J M Sneider, J T Roehrig, T M Woodward, D W Trent, Attenuation of Venezuelan equine encephalitis virus strain TC-83 is encoded by the 5'-noncoding region and the E2 envelope glycoprotein. Journal of Virology. ,vol. 67, pp. 1269- 1277 ,(1993) , 10.1128/JVI.67.3.1269-1277.1993
Udeni B. R. Balasuriya, Eric J. Snijder, Hans W. Heidner, Jianqiang Zhang, Jessika C. Zevenhoven-Dobbe, Josh D. Boone, William H. McCollum, Peter J. Timoney, N. James MacLachlan, Development and characterization of an infectious cDNA clone of the virulent Bucyrus strain of Equine arteritis virus. Journal of General Virology. ,vol. 88, pp. 918- 924 ,(2007) , 10.1099/VIR.0.82415-0
W.H. McCollum, P.J. Timoney, L.A. Tengelsen, Clinical, virological and serological responses of donkeys to intranasal inoculation with the KY-84 strain of equine arteritis virus. Journal of Comparative Pathology. ,vol. 112, pp. 207- 211 ,(1995) , 10.1016/S0021-9975(05)80062-3
Yue Wang, Yajie Liang, Jun Han, Kelly M. Burkhart, Eric M. Vaughn, Michael B. Roof, Kay S. Faaberg, Attenuation of porcine reproductive and respiratory syndrome virus strain MN184 using chimeric construction with vaccine sequence Virology. ,vol. 371, pp. 418- 429 ,(2008) , 10.1016/J.VIROL.2007.09.032