Cloning and functional expression of ATA1, a subtype of amino acid transporter A, from human placenta.
作者： Haiping Wang , Wei Huang , Mitsuru Sugawara , Lawrence D. Devoe , Frederick H. Leibach
关键词: Protein primary structure 、 Peptide sequence 、 Molecular biology 、 cDNA library 、 Biology 、 Amino acid transporter 、 Biochemistry 、 Complementary DNA 、 Transmembrane domain 、 Amino acid 、 Glutamate aspartate transporter
摘要: This report describes the primary structure and functional characteristics of human ATA1, a subtype amino acid transport system A. The ATA1 cDNA was isolated from placental library. codes for protein 487 acids with 11 putative transmembrane domains. transporter mRNA ( approximately 9.0 kb) is expressed most prominently in placenta heart, but detectable level expression evident other tissues including brain. When heterologously mammalian cells, cloned mediates Na(+)-coupled A-specific model substrate alpha-(methylamino)isobutyric acid. process saturable Michaelis-Menten constant 0. 89 +/- 0.12 mM. Na(+):amino stoichiometry 1:1 as deduced Na(+)-activation kinetics. specific small short-chain neutral acids. gene located on chromosome 12.
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