Loss of the Endothelial Glycocalyx Links Albuminuria and Vascular Dysfunction

摘要: Patients with albuminuria and CKD frequently have vascular dysfunction but the underlying mechanisms remain unclear. Because endothelial surface layer, a meshwork of surface-bound loosely adherent glycosaminoglycans proteoglycans, modulates function, its loss could contribute to both renal systemic in proteinuric CKD. Using Munich-Wistar-Fromter (MWF) rats as model spontaneous albuminuric CKD, multiphoton fluorescence imaging single-vessel physiology measurements revealed that old MWF exhibited widespread layer parallel defects microvascular permeability water albumin, continuous mesenteric microvessels fenestrated glomerular microvessels. In contrast young rats, enzymatic disruption resulted neither additional nor changes permeability. Intravenous injection wheat germ agglutinin lectin adsorption onto significantly improved albumin Taken together, these results suggest links kidney disease dysfunction, providing potential therapeutic target for disease.