The 222- to 234-kilodalton latent nuclear protein (LNA) of Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) is encoded by orf73 and is a component of the latency-associated nuclear antigen.

摘要: Kaposi's sarcoma (KS)-associated herpesvirus or human 8 (KSHV/HHV8) is the likely cause of KS and primary effusion lymphomas body cavity-based (BCBLs). A latency-associated nuclear immunofluorescence antigen (LANA) (D. H. Kedes, E. Operskalski, M. Busch, R. Kohn, J. Flood, D. Ganem, Nat. Med. 2:918-924, 1996; S. Gao, L. Kingsley, Li, W. Zheng, C. Parravicini, Ziegler, Newton, Rinaldo, A. Saah, Phair, Detels, Y. Chang, P. Moore, 2:925-928, 1996) a 222- to 234-kDa protein (LNA) (S. Hoover, T. Spira, Parry, N. Engl. 335:233-241, have previously been described in BCBL cell lines by Western blotting techniques, respectively. To identify viral gene(s) encoding this antigen(s) we screened cDNA library from HBL-6 cells, B-cell lymphoma line persistently infected with KSHV/HHV8, patient sera. One set positive clones contained 3' end orf73, as well complete orf72 orfK13, another 5' orf73. Comparison sequences KSHV/HHV8 genomic sequence revealed splice event, occurring upstream Immunoaffinity purified antibodies recombinant carboxy-terminal fragment orf73-encoded showed characteristic speckled pattern LANA reacted LNA on blots. Expression full-length orf73 bacteria COS7 cells reproduced banding pattern. Immunohistochemistry cases nodular that orf73/LNA expressed nucleus spindle cells. These findings demonstrate encodes LNA, component LANA, tumor