Effects of hydrogen peroxide on basal and hormone-stimulated lipolysis in perifused rat fat cells in relation to the mechanism of action of insulin.
作者： S.A. Little , C. de Haën
DOI: 10.1016/S0021-9258(19)70390-1
关键词: Adipose tissue 、 Glucagon 、 Stimulation 、 Internal medicine 、 Mechanism of action 、 Ritodrine 、 Hormone 、 Insulin 、 Endocrinology 、 Lipolysis 、 Chemistry
摘要: Since insulin is known to stimulate intracellular hydrogen peroxide production in rat epididymal fat cells, the effects of exogenous on rates basal and hormone-stimulated lipolysis were investigated a perifusion system. H2O2 (60 microM) caused weak transient stimulation that did not interfere with subsequent activation by hormones. More importantly, stimulated submaximally ritodrine (10(-7) M) or glucagon (10(-9) was inhibited manner similar insulin, although slight differences time course noted. Ritodrine served as beta-adrenergic agonist resistant oxidative destruction H2O2. The inhibition reversible upon cessation These findings ruled out hormone cell death explanations for antilipolytic effect Like also 1-methyl-3-isobutylxanthine (4 X 10(-6) M)-stimulated lipolysis, but whereas dibutyryl-cAMP 10(-4) M), further enhanced it. add another case growing list adipocytes can be mimicked H2O2, they hint at site where mechanisms action two agents may differ.
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