Ultrasound‐mediated gene transfer (sonoporation) in fibrin‐based matrices: potential for use in tissue regeneration

摘要: It has been suggested that gene transfer into donor cells is an efficient and practical means of locally supplying requisite growth factors for applications in tissue regeneration. Here we describe, the first time, ultrasound-mediated system can non-invasively facilitate entrapped within fibrin-based matrices. Since enhanced using microbubbles, compared efficacy neutral cationic forms these reagents on ultrasound-stimulated process gel In doing so demonstrated beneficial effects associated with use microbubble preparations interact directly nucleic acid some cases, expression was increased two-fold matrices when microbubbles were microbubbles. addition, incorporating collagen fibrin gels yielded a 25-fold increase after application ultrasound to microbubble-containing We suggest this novel may non-invasive temporal spatial control gel-based purposes