Regulation of Chemokine Recognition by Site-Specific Tyrosine Sulfation of Receptor Peptides

摘要: Summary Sulfation of tyrosine is a common posttranslational modification secreted proteins that influences numerous physiological and pathological processes. Studies sulfation have been hindered by the difficulty introducing sulfate groups at specific positions peptides proteins. Here we report general strategy for synthesis containing sulfotyrosine one or more position(s). The approach provides substantial improvement in both yield convenience over existing methods. Using synthetic sulfopeptides derived from chemokine receptor CCR3, demonstrate enhances affinity eotaxin ∼7-fold than 28-fold, depending on which two adjacent residues sulfated. methodology will substantially enhance efforts to understand functional structural consequences protein sulfation.