Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites.
作者： Željka Jovanović , Aleksandra Radosavljević , Zorica Kačarević-Popović , Jasmina Stojkovska , Aleksandra Perić-Grujić
DOI: 10.1016/J.COLSURFB.2012.12.055
关键词: Simulated body fluid 、 Silver nanoparticle 、 MTT assay 、 Kinetics 、 Bioreactor 、 Chemistry 、 Nanocomposite 、 Nanotechnology 、 Biocompatibility 、 Nuclear chemistry 、 Rate-determining step
摘要: Abstract Silver/poly(N-vinyl-2-pyrrolidone) (Ag/PVP) nanocomposites containing Ag nanoparticles at different concentrations were synthesized using γ-irradiation. Cytotoxicity of the obtained was determined by MTT assay in monolayer cultures normal human immunocompetent peripheral blood mononuclear cells (PBMC) that either non-stimulated or stimulated to proliferate mitogen phytohemagglutinin (PHA), as well cervix adenocarcinoma cell (HeLa) cultures. Silver release kinetics and mechanical properties investigated under bioreactor conditions simulated body fluid (SBF) 37 °C. The silver monitored static conditions, two types bioreactors: perfusion bioreactors a with dynamic compression coupled SBF simulating vivo articular cartilage. Ag/PVP exhibited slight cytotoxic effects against PBMC estimated concentration 0.4 μmol dm−3, negligible variations observed amongst investigated. Studies indicated internal diffusion rate limiting step, statistically comparable results all conditions. However, slightly higher compared other systems indicating influence compression. Modelling revealed potentials for optimization particular applications wound dressings soft tissue implants.
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