Method and system for accurate alignment and registration of array for DNA sequencing

摘要: In a genome sequencing system and methodology, protocol is provided to achieve precise alignment accurate registration of an image planar array nanoballs subject optical analysis. Precise correcting for fractional offsets achieved by errors in subperiod x-y offset, scale rotation use minimization techniques Moire averaging. averaging, magnification intentionally set so that the pixel period imaging element noninteger multiple site period. Accurate providing pre-defined pseudo-random sets sites, herein deletion or reserved where are prevented from attachment substrate sites can be used pattern matching scheme as markers absolute location identification. Information extracted with high degree confidence it correlated known location, while at same time amount information packed on chip maximized.