Post-ionization of neutrals for ion mobility otofms identification of molecules and elements desorbed from surfaces

摘要: The present invention relates to a method and apparatus for ionizing neutral MALDI desorption plume, in particular, efficiently measuring the ionized plume when post-ionization techniques are combined with medium pressure MALDI-IM-oTOFMS instrument. Additionally, disclosure provides that simultaneously separates tissue-sample ions by IM-oTOFMS according their chemical family. After separation, directly compared created post-ionizing co-desorbed molecules second laser wherein is delayed few hundred microseconds. further novel approaches enhance analysis of ions, including use giant fullerene internal standards mass accuracy, ultraviolet (UV) declustering lasers generate intact peptides proteins, either which may be followed VUV generates identifiable structural fragments.