Ligand detection method
作者： Frank Karlsen
DOI:
关键词: Receptor 、 Nucleic acid molecule 、 Specific detection 、 Chemistry 、 Stereochemistry 、 Nucleic acid 、 Reagent 、 Ligand
摘要: There is disclosed a sensitive method for specific detection of ligands comprising the steps (a) contacting sample with reagents capable forming reagent complex, which complex comprises receptor specifically binding to said ligand and nucleic acid molecule; (b) detecting any complexes formed by part present in presence molecule amplifying region simultaneously products amplification reaction real-time.
lens.org 参考文章(28)
Prabhakara V. Choudary, Christopher M. Gooding, Method for detection of pathogens in food ,(1996)
Charles R. Cantor, Takeshi Sano, Cassandra L. Smith, Immuno-polymerase chain reaction system for antigen detection ,(1993)
Robert H. Davis, Herbert A. Marble, Strategy for the production of rna from immobilized templates ,(1994)
Edwin R. Hendrickson, Michael P. Perry, Sandra Fitzpatrick-Mcelligott, Richard C. Ebersole, A method for the amplification and detection of a nucleic acid fragment of interest ,(1997)
Eddie Carroll, Richard A. Martinelli, Sensitive immunoassays utilizing antibody conjugates with replicable dna templates ,(1996)
Michael S. Urdea, Nucleic acid-amplified immunoassay probes ,(1993)
V Ruzicka, W Marz, A Russ, W Gross, Immuno-PCR with a commercially available avidin system. Science. ,vol. 260, pp. 698- 699 ,(1993) , 10.1126/SCIENCE.8480182
Edwin R. Hendrickson, Tina M. Hatfield Truby, Rolf D. Joerger, William R. Majarian, Richard C. Ebersole, High sensitivity multianalyte immunoassay using covalent DNA-labeled antibodies and polymerase chain reaction Nucleic Acids Research. ,vol. 23, pp. 522- 529 ,(1995) , 10.1093/NAR/23.3.522
Günther Strunk, Tobias Ederhof, Machines for automated evolution experiments in vitro based on the serial-transfer concept Biophysical Chemistry. ,vol. 66, pp. 193- 202 ,(1997) , 10.1016/S0301-4622(97)00062-8
T Sano, C. Smith, C. Cantor, Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates. Science. ,vol. 258, pp. 120- 122 ,(1992) , 10.1126/SCIENCE.1439758