Lack of association between Chlamydia trachomatis infection and cervical cancer--Taq Man realtime PCR assay findings.
作者： Taghi Naserpour Farivar , Pouran Johari
DOI: 10.7314/APJCP.2012.13.8.3701
关键词: Pathology 、 Cervical cancer 、 TaqMan 、 DNA extraction 、 Cancer 、 Chlamydia trachomatis infection 、 Chlamydia trachomatis 、 Pathogenesis 、 Biology 、 Real-time polymerase chain reaction
摘要: Background: Cervical cancer is one of the most common cancers in developing countries and second type women globally. Several recent studies suggested a co factor role for Chlamydia trachomatis pathogenesis cervical cancer. This study aimed to evaluate existence C. DNA pathologic blocks patients with Materials methods: Seventy-six formaldehyde fixed paraffin embedded tissue specimens from histologically proven history as well 150 healthy peoples were included present study. Thin slices prepared selected followed by deparaffinization extraction; presence was examined Taq Man real-time PCR. Results: Our TaqMan real time PCR assay showed that there no DNA. Also, we found three positive among our control group. Conclusion: It seems based on results obtained study, association between
koreascience.or.kr参考文章(33)
R.R. Finan, U. Musharrafieh, W.Y. Almawi, Detection of Chlamydia trachomatis and herpes simplex virus type 1 or 2 in cervical samples in human papilloma virus (HPV)-positive and HPV-negative women Clinical Microbiology and Infection. ,vol. 12, pp. 927- 930 ,(2006) , 10.1111/J.1469-0691.2006.01479.X
M. Valadan, F. Yarandi, Z. Eftekhar, S. Darvish, M.S. Fathollahi, A. Mirsalehian, Chlamydia trachomatis and cervical intraepithelial neoplasia in married women in a Middle Eastern community. Eastern Mediterranean Health Journal. ,vol. 16, pp. 304- 307 ,(2010) , 10.26719/2010.16.3.304
Gerardo D. Deluca, José M. Alonso, Lilian Vicente, Mónica Albhom, Estela M. Chamorro, Eduardo Schelover, Héctor M. Marín, Infección por Chlamydia trachomatis y papilomavirus en mujeres con alteraciones citohistológicas de cuello uterino Medicina-buenos Aires. ,vol. 66, pp. 303- 306 ,(2006)
M ZEREU, C ZETTLER, E CAMBRUZZI, A ZELMANOWICZ, Herpes simplex virus type 2 and Chlamydia trachomatis in adenocarcinoma of the uterine cervix Gynecologic Oncology. ,vol. 105, pp. 172- 175 ,(2007) , 10.1016/J.YGYNO.2006.11.006
N. V. Savel’eva, Yu. E. Zagryadskaya, S. V. Klimashevskaya, V. F. Puzyrev, A. N. Burkov, A. P. Obryadina, T. I. Ulanova, Scanning diagnostically significant antigenic regions of major Chlamydia Trachomatis protein MOMP using series of overlapping recombinant proteins Molecular Genetics, Microbiology and Virology. ,vol. 24, pp. 133- 137 ,(2009) , 10.3103/S0891416809030057
Ping Du, Allison Lemkin, Brenda Kluhsman, Jin Chen, Robert E Roth, Alan MacEachren, Craig Meyers, John J Zurlo, Eugene J Lengerich, None, The roles of social domains, behavioral risk, health care resources, and chlamydia in spatial clusters of US cervical cancer mortality: not all the clusters are the same Cancer Causes & Control. ,vol. 21, pp. 1669- 1683 ,(2010) , 10.1007/S10552-010-9596-4
Thomas G. Magaldi, Laura L. Almstead, Stefania Bellone, Edward G. Prevatt, Alessandro D. Santin, Daniel DiMaio, Primary human cervical carcinoma cells require human papillomavirus E6 and E7 expression for ongoing proliferation. Virology. ,vol. 422, pp. 114- 124 ,(2012) , 10.1016/J.VIROL.2011.10.012
Koen D. Quint, Maurits N.C. de Koning, Daan T. Geraets, Wim G.V. Quint, Edyta C. Pirog, Comprehensive analysis of Human Papillomavirus and Chlamydia trachomatis in in-situ and invasive cervical adenocarcinoma. Gynecologic Oncology. ,vol. 114, pp. 390- 394 ,(2009) , 10.1016/J.YGYNO.2009.05.013
F Xavier Bosch, S de Sanjosé, None, The Epidemiology of Human Papillomavirus Infection and Cervical Cancer Disease Markers. ,vol. 23, pp. 213- 227 ,(2007) , 10.1155/2007/914823
R Najafipour, F Ahmadpour, TNaserpour Farivar, AA Pahlevan, P Johari, F Safdarian, MAslani Mehr, Assessment of Helicobacter Pylori Prevalence by Scorpion Real-Time PCR in Chronic Tonsillitis Patients Journal of Global Infectious Diseases. ,vol. 4, pp. 38- 42 ,(2012) , 10.4103/0974-777X.93760