Correlation between the neostriatal content of the 1-methyl-4-phenylpyridinium species and dopaminergic neurotoxicity following 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine administration to several strains of mice

摘要: In the present study we observed pronounced differences in capacity of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to induce dopaminergic neurotoxicity several strains mice. For example, there was no MPTP-induced decrement neostriatal dopamine content Ace Swiss-Webster mice and a 92% Taconic Farms C57 bl Several parameters which could possibly explain this differential sensitivity MPTP were studied. These include: 1) monoamine oxidase-B (MAO-B) activity; 2) synaptosomes prepared from mouse accumulate 1-methyl-4-phenylpyridinium (MPP+), major metabolite formed via oxidation by MAO-B; 3) MPP+ after administration There significant Km values for MAO-B neostriatum among examined. Neostriatal Vmax differed somewhat strains, with low 2915 +/- 172 nmol/g tissue per hr (CD-1 Charles River) high 3884 203 (C57 Farms). However, did not correlate significantly relative MPTP. MPP+. Studies on metabolism peripheral revealed that (P less than .01) positive correlation between their administration.(ABSTRACT TRUNCATED AT 250 WORDS)