Identification of pathogen-responsive regions in the promoter of a pepper lipid transfer protein gene (CALTPI) and the enhanced resistance of the CALTPI transgenic Arabidopsis against pathogen and environmental stresses
作者： Ho Won Jung , Ki Deok Kim , Byung Kook Hwang
DOI: 10.1007/S00425-004-1461-9
关键词: Plant disease resistance 、 Gene 、 Pseudomonas syringae 、 Promoter 、 Genetics 、 Genetically modified crops 、 Genetic transfer 、 Transgene 、 Arabidopsis 、 Molecular biology 、 Biology
摘要: The 5′ flanking region of the CALTPI gene, which encodes a basic lipid transfer protein, was isolated and characterized from genomic DNA Capsicum annuum. Four different regions promoter sequence gene were fused to β-glucuronidase (GUS) coding region. In an Agrobacterium-mediated transient expression assay, transcriptional activations deletions examined in tobacco leaves after infection with Pseudomonas syringae pv. tabaci, treatment ethylene salicylic acid. −808 bp exhibited full activity. W-box ERE-box elements, are essential for induction by all signals, localized between −555 −391 upstream translation initiation site. A transgene then introduced under control 35S into Arabidopsis ecotype Col-0. Transgenic lines expressing developed rapidly compared wild-type plants, indicating that may be involved plant development. Overexpression enhanced resistance against P. tomato Botrytis cinerea. transgenic plants also showed high levels tolerance NaCl drought stresses at various vegetative growth stages. No transcription PR-1, PR-2, PR-5, thionin, RD29A genes observed untreated leaf tissues plants. pathogen environmental correlated gene.
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