Globular embryo-like structures and highly efficient thidiazuron-induced multiple shoot formation in saffron (Crocus sativus L.)

摘要: Saffron (Crocus sativus L.) is a monocotyledonous plant propagated via corms, but recently several alternative methods have been reported. To find the conditions suitable for saffron shoot formation from effect of different concentrations growth regulatory cytokinins N6-benzyladenine (BA) and N-phenyl-1, 2,3-thidiazol-5-ylurea, commonly known as thidiazuron (TDZ), were compared. In all corm explants, an average 39.5 ± 5.1 shoots per induced by 4.54 μM TDZ, whereas only 3.6-11.4% BA. The outstanding result in stage generation globular, translucent structures that are morphologically similar to globular embryos. optimize regeneration adventitious obtained TDZ treatment, transferred MS B5 media supplemented with combinations NAA highest rate developing was observed medium containing 2.22 2.68 With optimized hormonal conditions, 19.55 5.75 3.18 1.5 roots explants obtained. Based on this experiment, simple, new efficient protocol presented produce numerous plants saffron.