Global identification of androgen response elements.

摘要: Chromatin immunoprecipitation (ChIP) is an invaluable tool in the study of transcriptional regulation. ChIP methods require both a priori knowledge regulators which are important for given biological system and high-quality specific antibodies these targets. The androgen receptor (AR) known to play essential roles male sexual development, prostate cancer function many other AR-expressing cell types (e.g. neurons myocytes). As ligand-activated transcription factor AR also represents endogenous, inducible biology. Therefore, studies can make use treatment contrast experiments define its To date several have mapped binding sites using combination with genome tiling microarrays (ChIP-chip) or direct sequencing (ChIP-seq), mainly lines varying degrees genomic coverage. These provided new insights into DNA sequences bind, identified cooperating factors, thousands potential regulated genes processes by AR. However, further will be required fully characterise dynamics AR-regulated programme, map occupancy different complexes result output delineate networks downstream