Long-term persistence and bacterial transformation potential of transplastomic plant DNA in soil

摘要: The long-term physical persistence and biological activity of transplastomic plant DNA (transgenes contained in the chloroplast genome) either purified added to soil or naturally released by decaying tobacco leaves was determined. Soil microcosms were amended with incubated for up 4 years. Total extracted from number transgenes (aadA, which confers resistance both spectinomycin streptomycin) quantified quantitative PCR. these assessed transformation bacterial strain Acinetobacter sp. BD413(pBAB2) vitro. While proportion recovered increased increasing amount added, rapidly degraded over time. decreased about 10,000 fold within 2 weeks. Data reveal, however, that a small fraction escaped degradation. Transgene sequences still detected after years assays showed remained biologically active could transform competent cells BD413(pBAB2). approach presented here (based on PCR 50% gene) persisting environment time provided new insights into fate transgenic soil.