Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from Sargassum aquifolium.

摘要: Intensive efforts have been undertaken in the fields of prevention, diagnosis, and therapy lung cancer. Fucoidans exhibit a wide range biological activities, which are dependent on degree sulfation, sulfation pattern, glycosidic branches, molecular weight fucoidan. The determination oversulfation fucoidan its effect anti-lung cancer activity related signaling cascades is challenging. In this investigation, we used previously developed (SCA), served as native fucoidan, to generate two oversulfated derivatives (SCA-S1 SCA-S2). SCA, SCA-S1, SCA-S2 showed differences compositions had characteristic structural features by Fourier transform infrared (FTIR) nuclear magnetic resonance (NMR) analyses. anticancer properties against human carcinoma A-549 cells were analyzed terms cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, underlying cascades. Our findings indicate that promotes apoptosis mechanism may involve Akt/mTOR/S6 pathway. Further vivo research needed establish precise whereby mitigates progression