Evaluation of Striatal Dopamine Transporter Function in Rats by in Vivo β-[123I]CIT Pinhole SPECT

摘要: Striatal dopamine transporter (DAT) function was evaluated in rats by vivo SPECT-MRI coregistration using the radioligand 2-beta-carbomethoxy-3-beta-(4-[123I]iodophenyl)tropane (beta-[123I]CIT). The reconstructed transaxial resolution of 3.5 mm full width at half-maximum and system sensitivity 0.081 c/s/kBq a 2.0-mm pinhole collimator aperture provided adequate spatial detail sufficient for imaging striatal beta-[123I]CIT uptake. SPECT images, coregistered onto MRI template, showed high accuracy coronal transverse planes (maximum mismatch 1.3 mm). Following estimation binding equilibrium healthy rat striatum, we 6-hydroxydopamine-induced loss DAT correlated these findings with dopaminergic cell counts substantia nigra pars compacta TH immunohistochemistry. A subtotal unilateral deficit detected all animals which significantly counting remaining neurons. provides powerful widely available tool investigations function. In contrast to classical autoradiography, present method will be helpful dynamic changes neurotransmission CNS virtue serial study designs. Depending on ligand availability, wide range other receptors may imaged as well presented technique.