Soil pH and biome are both key determinants of soil archaeal community structure

摘要: Abstract The mechanisms underlying community composition and diversity of soil archaea are poorly understood. We compared both total ammonia oxidizing (AOA) using 16S rRNA amoA genes pyrosequencing respectively, in two different biomes: tropics (Malaysia), temperate (Korea Japan). Despite differences characteristics these biomes, we found that at the broad taxonomic level dominant archaeal lineages same, except certain instances (16S gene: group 1.1a Thaumarchaeota ; Nitrososphaera Nitrosotalea lineages). However, OTU level, AOA communities showed biome-specific patterns, indicating lower levels biome also important. In a negative correlation with pH, but hump-shaped curve for diversity, peaking ∼pH 6.0. Within each biome, pH emerged as delimiting factor determining variation AOA. Communities from clustered separately, even analogous levels. At shared OTUs did occur approximately same range biomes. closely related respectively tended to co-occur, suggesting evolutionary terms have conserved very similar ecological requirements. This predictability strongly suggests assembly has deterministic aspect. Overall, our findings emphasize large extent predictable structured by chemical environment, especially pH.