Differences in the efficiency of reductive activation of methionine synthase and exogenous electron acceptors between the common polymorphic variants of human methionine synthase reductase.

摘要: Methionine synthase reductase (MSR) catalyzes the conversion of inactive form human methionine to active state enzyme. This reaction is paramount physiological importance since an essential enzyme that plays a key role in and folate cycles. A common polymorphism MSR has been identified (66A → G) leads replacement isoleucine with at residue 22 allele frequency 0.5. Another 524C T, which substitution serine 175 leucine, but its not known. The I22M genetic determinant for mild hyperhomocysteinemia, risk factor cardiovascular disease. In this study, we have examined kinetic properties M22/S175 I22/S175 I22/L175 pairs variants. EPR spectra semiquinone forms variants are indistinguishable exhibit isotropic signal g = 2.00. addition, the...