Cerebrospinal fluid lipoperoxides quantified by liquid chromatography, and determination of reference values.

摘要: Cerebrospinal fluid lipoperoxides, measured as the malondialdehyde-thiobarbituric acid (MDA-TBA) adduct, were quantified by adapting plasma liquid-chromatographic method of Wong et al. (Clin Chem 1987;33:214-20) to cerebrospinal fluid. Reference values for spinal specimens from 91 adults, ages 17 95 y, and 37 children, 8 d determined. Their concentrations not significantly different (P = 0.222), adults having a mean (and SD) 0.11 (0.06) mumol children 0.10 (0.04) MDA per liter. ranges 0.02-0.26 0.04-0.21 liter, respectively. We found in be increased several central nervous system disorders, including seizures, cerebral infarction, alcoholic encephalopathy, and, perhaps, prematurity. The presence other thiobarbituric acid-reactive substances stresses importance using highly specific techniques when lipoperoxides are body fluids.