Patterns of myocardial histogenesis as revealed by mouse chimeras

摘要: In order to study the pattern of clonal myocyte distribution during mammalian heart development, we have exploited embryo aggregation chimeras using, as cellular markers, an enhanced jellyfish green fluorescent protein (eGFP) transgene and a desmin-promoter-driven, nuclear-localized beta-galactosidase (nlacZ) knock-in. neonatal, weanling, adult chimeric atria ventricles, irregularly formed patches various sizes rather than highly dispersed cardiomyocytes were observed. Most smaller single found in spatial neighborhood large patches. This indicated largely coherent growth myocardial histogenesis combined with tangential displacement or active migration myocytes. The patterns ventricular walls simpler those septum atria. heart, volumes devoid eGFP-positive lack secondary immigration blood-borne stem cells into myocardium. oligoclonal expansions revealed this work might be helpful detecting analyzing cell-lineage-based pathological processes heart.