The CpxRA two-component system contributes to Legionella pneumophila virulence.
作者： Jennifer R. Tanner , Laam Li , Sébastien P. Faucher , Ann Karen C. Brassinga
DOI: 10.1111/MMI.13365
关键词: Secretion 、 Biology 、 Transcriptome 、 Mutant 、 Legionella pneumophila 、 Virulence factor 、 Virulence 、 Effector 、 Intracellular 、 Microbiology
摘要: The bacterium Legionella pneumophila is capable of intracellular replication within freshwater protozoa as well human macrophages, the latter which results in serious pneumonia Legionnaires' disease. A primary factor involved these host cell interactions Dot/Icm Type IV secretion system responsible for translocating effector proteins needed to establish and maintain bacterial replicative niche. Several regulatory factors have been identified control expression effectors, one CpxRA two-component system, suggesting essentiality virulence. In this study, we generated cpxR, cpxA cpxRA in-frame null mutant strains further delineate role survival We found that cpxR essential Acanthamoeba castellanii, but not U937-derived macrophages. Transcriptome analysis revealed regulates a large number virulence-associated including effectors II secreted substrates. Furthermore, were more sodium resistant than parental strain Lp02, reaches maximal levels during postexponential phase. Taken together, our findings suggest key contributor L. virulence via regulation.
sci-hub.se 参考文章(111)
Gil Segal, The Legionella pneumophila Two-Component Regulatory Systems that Participate in the Regulation of Icm/Dot Effectors Current Topics in Microbiology and Immunology. ,vol. 376, pp. 35- 52 ,(2013) , 10.1007/82_2013_346
Andrew Bryan, Zachary D. Abbott, Michele S. Swanson, Constructing Unmarked Gene Deletions in Legionella pneumophila Methods in Molecular Biology. ,vol. 954, pp. 197- 212 ,(2013) , 10.1007/978-1-62703-161-5_10
Nicholas P. Cianciotto, Type II secretion and Legionella virulence. Current Topics in Microbiology and Immunology. ,vol. 376, pp. 81- 102 ,(2013) , 10.1007/82_2013_339
Aline Kessler, Ursula Schell, Tobias Sahr, André Tiaden, Christopher Harrison, Carmen Buchrieser, Hubert Hilbi, The Legionella pneumophila orphan sensor kinase LqsT regulates competence and pathogen–host interactions as a component of the LAI‐1 circuit Environmental Microbiology. ,vol. 15, pp. 646- 662 ,(2013) , 10.1111/J.1462-2920.2012.02889.X
Jacqueline R. Hellinga, Rafael A. Garduño, Jay D. Kormish, Jennifer R. Tanner, Deirdre Khan, Kristyn Buchko, Celine Jimenez, Mathieu M. Pinette, Ann Karen C. Brassinga, Identification of vacuoles containing extraintestinal differentiated forms of Legionella pneumophila in colonized Caenorhabditis elegans soil nematodes MicrobiologyOpen. ,vol. 4, pp. 660- 681 ,(2015) , 10.1002/MBO3.271
Peter De Wulf, Ohsuk Kwon, E. C. C. Lin, The CpxRA signal transduction system of Escherichia coli: growth-related autoactivation and control of unanticipated target operons. Journal of Bacteriology. ,vol. 181, pp. 6772- 6778 ,(1999) , 10.1128/JB.181.21.6772-6778.1999
T L Raivio, T J Silhavy, Transduction of envelope stress in Escherichia coli by the Cpx two-component system. Journal of Bacteriology. ,vol. 179, pp. 7724- 7733 ,(1997) , 10.1128/JB.179.24.7724-7733.1997
Olga Vassieva, Andrei Osterman, Alexei E. Kazakov, Ross A. Overbeek, Mikhail S. Gelfand, Bioinformatics classification and functional analysis of PhoH homologs. in Silico Biology. ,vol. 3, pp. 3- 15 ,(2003)
Peter De Wulf, Brian J. Akerley, E. C. C. Lin, Presence of the Cpx system in bacteria. Microbiology. ,vol. 146, pp. 247- 248 ,(2000) , 10.1099/00221287-146-2-247
Laura M. Hales, Howard A. Shuman, The Legionella pneumophila rpoS Gene Is Required for Growth within Acanthamoeba castellanii Journal of Bacteriology. ,vol. 181, pp. 4879- 4889 ,(1999) , 10.1128/JB.181.16.4879-4889.1999