Effects and mechanisms of waterborne copper exposure influencing ovary development and related hormones secretion in yellow catfish Pelteobagrus fulvidraco.
作者： Li-Han Zhang , Zhi Luo , Yu-Feng Song , Xi Shi , Ya-Xiong Pan
DOI: 10.1016/J.AQUATOX.2016.07.014
关键词: Sexual differentiation 、 Steroidogenic acute regulatory protein 、 Aromatase 、 Hormone 、 Cholesterol side-chain cleavage enzyme 、 Catfish 、 Biology 、 Ovary 、 Steroidogenic factor 1 、 Endocrinology 、 Internal medicine
摘要: The present study was conducted to determine the effects and mechanism of waterborne copper (Cu) exposure influencing ovary development related hormones secretion in yellow catfish Pelteobagrus fulvidraco. To this end, two experiments were conducted. In Exp. 1, partial cDNA sequences three steroidogenesis-related genes (androgen receptor (ar), steroidogenic factor 1 (sf-1) acute regulatory protein (star)) firstly characterized from P. predicted amino acid for fulvidraco ar, sf-1 star contained main structural features characteristic other species. 2, exposed Cu concentrations (control, 30μg/l 60μg/l, respectively) 56days. Sampling occurred on day 28 56, respectively. On 28, levels serum sex-steroid (FSH LH) mRNA (3β-hsd, cyp11a1, cyp17, cyp19a, star) significantly increased 30μg Cu/l. immunohistochemical analysis showed positive reaction ER, VTG aromatase low dose group. These indicated that relative short-term exposure, beneficial. contrast, 60μg Cu/l reduced FSH, LH, E2 P, ovarian 20β-hsd, cyp19a erα concentration gonadotropins sex hormones, down-regulated genes, indicating long-term had toxic effect hormone For first time, our cloned fulvidraco, demonstrated synthesis dose- time-dependent manner which will help understand Cu-induced reproductive toxicity at both transcriptional fish.
sci-hub.se 参考文章(87)
Li-Han Zhang, Xiao-Ying Tan, Kun Wu, Mei-Qin Zhuo, Yu-Feng Song, Qing-Ling Chen, Regulation and mechanism of leptin on lipid metabolism in ovarian follicle cells from yellow catfish Pelteobagrus fulvidraco General and Comparative Endocrinology. ,vol. 222, pp. 116- 123 ,(2015) , 10.1016/J.YGCEN.2015.06.008
Qi-Liang Chen, Zhi Luo, Cai-Xia Liu, Jia-Lang Zheng, Qing-Ling Zhu, Wei Hu, Mei-Qin Zhuo, Effects of waterborne copper exposure on carnitine composition, kinetics of carnitine palmitoyltransferases I (CPT I) and mRNA levels of CPT I isoforms in yellow catfish Pelteobagrus fulvidraco. Chemosphere. ,vol. 139, pp. 349- 357 ,(2015) , 10.1016/J.CHEMOSPHERE.2015.06.075
H. Zenkevičs, V. Vose, A. Būcena, Copper ions influence sturgeon gonadotropin functions. Proceedings of the Latvian Academy of Sciences. Section B, Natural, Exact and Applied Sciences. ,vol. 54, pp. 46- 47 ,(2000)
Pulak R. Manna, Cloyce L. Stetson, Andrzej T. Slominski, Kevin Pruitt, Role of the steroidogenic acute regulatory protein in health and disease. Endocrine. ,vol. 51, pp. 7- 21 ,(2016) , 10.1007/S12020-015-0715-6
P. Garcia-Morales, M. Saceda, N. Kenney, N. Kim, D.S. Salomon, M.M. Gottardis, H.B. Solomon, P.F. Sholler, V.C. Jordan, M.B. Martin, Effect of cadmium on estrogen receptor levels and estrogen-induced responses in human breast cancer cells. Journal of Biological Chemistry. ,vol. 269, pp. 16896- 16901 ,(1994) , 10.1016/S0021-9258(19)89474-7
Jo Vandesompele, Katleen De Preter, Filip Pattyn, Bruce Poppe, Nadine Van Roy, Anne De Paepe, Frank Speleman, Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes Genome Biology. ,vol. 3, pp. 1- 12 ,(2002) , 10.1186/GB-2002-3-7-RESEARCH0034
Yoshitaka Nagahama, Michiyasu Yoshikuni, Masakane Yamashita, Toshinobu Tokumoto, Yoshinao Katsu, Regulation of oocyte growth and maturation in fish Current Topics in Developmental Biology. ,vol. 30, pp. 103- 145 ,(1995) , 10.1016/S0070-2153(08)60565-7
Michael C. Henson, P. Jorge Chedrese, Endocrine disruption by cadmium, a common environmental toxicant with paradoxical effects on reproduction. Experimental Biology and Medicine. ,vol. 229, pp. 383- 392 ,(2004) , 10.1177/153537020422900506
Rie Goto-Kazeto, Katherine E. Kight, Yonathan Zohar, Allen R. Place, John M. Trant, Localization and expression of aromatase mRNA in adult zebrafish. General and Comparative Endocrinology. ,vol. 139, pp. 72- 84 ,(2004) , 10.1016/J.YGCEN.2004.07.003
Feng He, Haishen Wen, Dahui Yu, Jifang Li, Bao Shi, Caifang Chen, Jiaren Zhang, Guoxiong Jin, Xiaoyan Chen, Dan Shi, Yanping Yang, Single nucleotide polymorphism of FSHβ gene associated with reproductive traits in Japanese flounder (Paralichthys olivaceus) Journal of Ocean University of China. ,vol. 9, pp. 395- 398 ,(2010) , 10.1007/S11802-010-1776-Z