Control of head morphogenesis in an invertebrate asexually produced larva-like bud (Cassiopea andromeda; Cnidaria: Scyphozoa)

摘要: Scyphopolyps of Cassiopea andromeda propagate asexually by forming larva-like buds which separate from the parent in a developmentally quiescent state. These metamorphose into sessile polyps when exposed to specific biogenic, chemical inducers. Morphogenesis transversely dissected indicates presence pattern-determining signals; whereas basal bud fragments may still form complete scyphistoma apical develop spontaneously absence an inducer polyp head without stalk and foot. Based on these findings Neumann (dissertation, Cologne University, 1980) postulated head-inhibiting signal is released at pole inhibits formation end. Contrary this hypothesis dissection itself might induce development structures. The present study deals with control C. andromeda. It concentrates two points, namely inhibitor involvement compounds known act during metamorphosis (the enzyme protein kinase C Z-GPGGPA). We found that compared intact incised reached intermediate stage development. This confirms Neumann's hypothesis. Consequently we focused mode action nature Our results indicate be included one six pooled fractions isolated homogenate via gel filtration Sephadex G-50 column. supposed water-soluble have molecular weight 850-1,500 Da. Furthermore prove not promoted metamorphosis-inducer Z-GPGGPA but prevented inhibitors psychosine, chelerythrine RO-32-0432 showing process.