Histopathologic correlate of hypointense lesions on T1-weighted spin-echo MRI in multiple sclerosis

摘要: Postmortem unfixed whole brains from five multiple sclerosis (MS) patients were examined by MRI using a T2- and T1-weighted spin-echo (SE) sequence histology to investigate the histopathologic characteristics of hypointense lesions on SE MR images. The degree hypointensity was scored semiquantitatively two blinded observers in reference normal-appearing white matter. Signal intensities matter measured obtain contrast ratios. Hematoxylin-eosin stain used assess matrix destruction (decrease density neuropil) cellularity lesion, Kluver-Barrera for demyelination, Bodian axonal density, immunostaining glial fibrillary acid protein reactive astrocytes gliosis. Nineteen selected analysis. Nearly all compatible with chronic MS plaque: hypocellularity, absence myelinated axons, presence astrocytes. Contrast ratios highly correlated (R = -0.90; p 0.001). A trend found 0.45; 0.052). We conclude that, our limited sample, seen images are associated histopathologically severe tissue destruction, including loss. Our results need be substantiated larger study more varied patient material evaluate use as surrogate marker persistent deficit patients.