Frontline Science: Two flavonoid compounds attenuate allergic asthma by regulating epithelial barrier via G protein-coupled estrogen receptor: Probing a possible target for allergic inflammation.

摘要: Allergic asthma is a common chronic lung inflammatory disease and seriously influences public health. We aim to investigate the effects of formononetin (FMN) calycosin (CAL), 2 flavonoids in Radix Astragali, on allergic elucidate possible therapeutic targets. A house dust mite (HDM)-induced mouse model TNF-α Poly(I:C) co-stimulated human bronchial epithelial cell line (16HBE) were performed respectively vivo vitro. The role G protein-coupled estrogen receptor (GPER) was explored by its agonist, antagonist, or GPER small interfering RNA (siGPER). E-cadherin, occludin, detected western blotting, immunohistochemistry, immunofluorescence. barrier integrity assessed trans-epithelial electric resistance (TEER). Cytokines examined enzyme-linked immunosorbent assay (ELISA). results showed that attenuated pulmonary inflammation hyperresponsiveness asthmatic mice. These significantly inhibited thymic stromal lymphopoietin (TSLP), increased occludin restored E-cadherin TSLP not interfered ICI182780 (estrogen antagonist), while blocked G15 (GPER antagonist). Furthermore, compared with PPT (ERα agonist) DPN (ERβ agonist), G1 TSLP, up-regulated E-cadherin. siGPER TEER assays suggested pivotal for integrity. Finally, inflammation, which could be abolished G15. Our data demonstrated Astragali alleviate protecting via regulating GPER, activating might strategy against inflammation.