Ginkgolide B Inhibits JAM-A, Cx43, and VE-Cadherin Expression and Reduces Monocyte Transmigration in Oxidized LDL-Stimulated Human Umbilical Vein Endothelial Cells
作者： Xueqing Liu , Wenjia Sun , Yanyang Zhao , Beidong Chen , Wei Wu
DOI: 10.1155/2015/907926
关键词: VE-cadherin 、 Monocyte 、 Western blot 、 Cell biology 、 Cadherin 、 Ginkgolide 、 Vascular permeability 、 Umbilical vein 、 Connexin 、 Biology
摘要: Aim. To investigate the effect of ginkgolide B on junction proteins and reduction monocyte migration in oxidized low-density lipoprotein- (ox-LDL-) treated endothelial cells. Methods. Human umbilical vein cells (HUVECs) were used present study. Immunofluorescence Western blot performed to determine expression junctional adhesion molecule-A (JAM-A), connexin 43 (Cx43), vascular cadherin (VE-cadherin). Monocyte was detected by Transwell assay. Results. ox-LDL stimulation increased JAM-A 35%, Cx43 24%, VE-cadherin 37% HUVECs. Ginkgolide (0.2, 0.4, 0.6 mg/mL) dose-dependently abolished these proteins. The transmigration experiments showed that level sixfold higher ox-LDL-treated group than control group. (0.6 mg/mL) nearly completely migration. Both LY294002 suppressed Akt phosphorylation These results suggest B-induced inhibition protein is associated with blockade PI3K/Akt pathway. Conclusion. reduced induced ox-LDL. may improve permeability atherosclerosis.
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