Wound-induced expression of DEFECTIVE IN ANTHER DEHISCENCE1 and DAD1-like lipase genes is mediated by both CORONATINE INSENSITIVE1-dependent and independent pathways in Arabidopsis thaliana

摘要: Key message Endogenous JA production is not neces- sary for wound-induced expression of JA-biosynthetic lipase genes such as DAD1 in Arabidopsis. However, the JA-Ile receptor COI1 often required their JA- independent induction. Abstract Wounding a serious event plants that may result from insect feeding and increase risk pathogen infection. Wounded produce high amounts jas- monic acid (JA), which triggers resistance genes. We focused on tran- scriptional regulation DEFECTIVE IN ANTHER DEHISCENCE1 six its homologs including DON- GLE (DGL) Arabidopsis, encode lipases involved biosynthesis. Plants constitutively expressing accumulated higher amount than control after wounding, indicating these contributes to determining levels. found DAD1, DGL, other DAD1- LIKE LIPASE (DALL) induced upon wounding. Some DALLs were also expressed unwounded leaves. Further experiments using response mutants revealed wound induction regulated by several distinct pathways. most DALL4 fully without relying endogenous pro- duction only partly affected deficiency, positive feedback necessary DGL CORONATINE INSENSITIVE1 (COI1) expression, suggesting molecule might act regulator COI1. Wound DALL1, DALL2, DALL3 did require This differential explain functions at different time points