Selection of Rattus norvegicus oocytes for in vitro maturation by brilliant cresyl blue staining.
作者： Diego Duarte Alcoba , Bianca Letícia da Rosa Braga , Nathallie Louise Sandi-Monroy , Letícia Auler Proença , Rui Fernando Felix Lopes
DOI: 10.1017/S0967199411000463
关键词: Predictive value 、 Staining 、 Diagnostic test 、 Andrology 、 Brilliant cresyl blue 、 In vitro maturation 、 Chemistry 、 Incubation period
摘要: The objective of this work was to evaluate the rate meiosis resumption and nuclear maturation rat ( Rattus norvegicus ) oocytes selected for in vitro (IVM) after staining cumulus–oocyte complexes (COCs) with blue cresyl brilliant (BCB) using different protocols: exposure 30, 60 or 90 min at 26 μM BCB (Experiment 1), 13, 20 2). In Experiment 1, selection exposed found be most suitable, as rates + group n = 35/61; 57.37%) were closest observed control (not exposed) 70/90; 77.77%) statistically higher than values − 3/41; 7.32%). Additionally, more effective evaluation diagnostic tests (sensitivity negative predictive value 100%) COCs min. 2, 13 presented 57/72; 72.22%) similar 87/105; 82.86%) other concentration groups. However, results analysis between also 28/91; 30.78%) when compared 3/62; 4.84%) 3/61; 4.92%) BCB. oocytes. had a lower Thus, our best by obtained μM, an incubation period
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