Ca2+ ionophore A23187 inhibits ATP generation reducing mouse sperm motility and PKA-dependent phosphorylation.

作者: Qiangzhen Yang , Yi Wen , Lirui Wang , Zijun Peng , Ranna Yeerken

DOI: 10.1016/J.TICE.2020.101381

关键词: Citric acid cycleCell biologyBiosynthesisBiologyFlux (metabolism)MitochondrionSperm motilityElectron transport chainPhosphorylationSperm

摘要: … The ATP assay kits were used to measure ATP levels of three sperm (NC, C and A23187) … directly regulate ATP generation, therefore we tested ATP contents observing that ATP were …

参考文章(37)
Atthaboon Watthammawut, Monsicha Somrit, Somluk Asuvapongpatana, Wattana Weerachatyanukul, Enhancement of trypsin-like enzymes by A23187 ionophore is crucial for sperm penetration through the egg vestment of the giant freshwater prawn Cell and Tissue Research. ,vol. 362, pp. 643- 652 ,(2015) , 10.1007/S00441-015-2226-4
Felipe A. Navarrete, Francisco A. García-Vázquez, Antonio Alvau, Jessica Escoffier, Dario Krapf, Claudia Sánchez-Cárdenas, Ana M. Salicioni, Alberto Darszon, Pablo E. Visconti, Biphasic role of calcium in mouse sperm capacitation signaling pathways. Journal of Cellular Physiology. ,vol. 230, pp. 1758- 1769 ,(2015) , 10.1002/JCP.24873
G Wiegand, S J Remington, Citrate Synthase: Structure, Control, and Mechanism Annual Review of Biophysics and Biophysical Chemistry. ,vol. 15, pp. 97- 117 ,(1986) , 10.1146/ANNUREV.BB.15.060186.000525
P. E. Visconti, Dieyun Pan, G. S. Kopf, G. D. Moore, S. A. Connors, J. L. Bailey, P. Olds-Clarke, P. Leclerc, Capacitation of mouse spermatozoa. II. Protein tyrosine phosphorylation and capacitation are regulated by a cAMP-dependent pathway. Development. ,vol. 121, pp. 1139- 1150 ,(1995) , 10.1242/DEV.121.4.1139
Ka Chen, Qianyong Zhang, Jian Wang, Fengjin Liu, Mantian Mi, Hongxia Xu, Fang Chen, Kaihong Zeng, Taurine protects transformed rat retinal ganglion cells from hypoxia-induced apoptosis by preventing mitochondrial dysfunction. Brain Research. ,vol. 1279, pp. 131- 138 ,(2009) , 10.1016/J.BRAINRES.2009.04.054
Dario Krapf, Enid Arcelay, Eva V. Wertheimer, Archana Sanjay, Stephen H. Pilder, Ana M. Salicioni, Pablo E. Visconti, Inhibition of Ser/Thr Phosphatases Induces Capacitation-associated Signaling in the Presence of Src Kinase Inhibitors Journal of Biological Chemistry. ,vol. 285, pp. 7977- 7985 ,(2010) , 10.1074/JBC.M109.085845
R.A.P. Harrison, Rapid PKA-catalysed phosphorylation of boar sperm proteins induced by the capacitating agent bicarbonate. Molecular Reproduction and Development. ,vol. 67, pp. 337- 352 ,(2004) , 10.1002/MRD.20028
A. Ruknudin, I. A. Silver, Ca2+ uptake during capacitation of mouse spermatozoa and the effect of an anion transport inhibitor on Ca2+ uptake. Molecular Reproduction and Development. ,vol. 26, pp. 63- 68 ,(1990) , 10.1002/MRD.1080260110
Ellie Himschoot, Tom Beeckman, Jiří Friml, Steffen Vanneste, Calcium is an organizer of cell polarity in plants Biochimica et Biophysica Acta. ,vol. 1853, pp. 2168- 2172 ,(2015) , 10.1016/J.BBAMCR.2015.02.017
Enid Arcelay, Ana M. Salicioni, Eva Wertheimer, Pablo E. Visconti, Identification of proteins undergoing tyrosine phosphorylation during mouse sperm capacitation. The International Journal of Developmental Biology. ,vol. 52, pp. 463- 472 ,(2008) , 10.1387/IJDB.072555EA