Overlooked: Extrachromosomal DNA and Their Possible Impact on Whole Genome Sequencing.
作者： Reinhard H Dennin ,
关键词: Genome 、 Chromosome 、 Extrachromosomal DNA 、 Chromosomal dna 、 Base pair 、 Genetics 、 Whole genome sequencing 、 DNA 、 Human genome 、 Medicine
摘要: Extrachromosomal (ec) DNA in eukaryotic cells has been known for decades. The structures described range from linear double stranded (ds) to circular dsDNA, distinct mitochondrial (mt) DNA. sizes of forms are some hundred base pairs (bp) up more than 150 kbp. number molecules per cell ranges several a thousand. Semi-quantitative determinations dsDNA show proportions as high percentages the total cell. These ecDNA fractions harbor sequences that be present chromosomal (chrDNA) too. Sequencing projects on, example human genome, have take into account which simultaneously ascertained; corrections cannot performed retrospectively. Concerning results sequencings derived extracted whole DNA: if contained therein not taken account, erroneous conclusions at level may result.
sci-hub.st 参考文章(51)
Sal'nokov Kv, Extrachromosomal DNA in mammalian cells Tsitologiia. ,vol. 32, pp. 1061- 1071 ,(1990)
Laura W. Dillon, Pankaj Kumar, Yoshiyuki Shibata, Yuh-Hwa Wang, Smaranda Willcox, Jack D. Griffith, Yves Pommier, Shunichi Takeda, Anindya Dutta, Production of Extrachromosomal MicroDNAs Is Linked to Mismatch Repair Pathways and Transcriptional Activity Cell Reports. ,vol. 11, pp. 1749- 1759 ,(2015) , 10.1016/J.CELREP.2015.05.020
Chris Tyler-Smith, Huanming Yang, Laura F. Landweber, Ian Dunham, Bartha M. Knoppers, Peter Donnelly, Elaine R. Mardis, Michael Snyder, Gil McVean, Where Next for Genetics and Genomics? PLOS Biology. ,vol. 13, pp. e1002216- ,(2015) , 10.1371/JOURNAL.PBIO.1002216
Maria S. Poptsova, Irina A. Il'icheva, Dmitry Yu. Nechipurenko, Larisa A. Panchenko, Mingian V. Khodikov, Nina Y. Oparina, Robert V. Polozov, Yury D. Nechipurenko, Sergei L. Grokhovsky, Non-random DNA fragmentation in next-generation sequencing Scientific Reports. ,vol. 4, pp. 4532- 4535 ,(2015) , 10.1038/SREP04532
Yoshiyuki Shibata, Pankaj Kumar, Ryan Layer, Smaranda Willcox, Jeffrey R. Gagan, Jack D. Griffith, Anindya Dutta, Extrachromosomal MicroDNAs and Chromosomal Microdeletions in Normal Tissues Science. ,vol. 336, pp. 82- 86 ,(2012) , 10.1126/SCIENCE.1213307
Dale W Garsed, Owen J Marshall, Vincent DA Corbin, Arthur Hsu, Leon Di Stefano, Jan Schröder, Jason Li, Zhi-Ping Feng, Bo W Kim, Mark Kowarsky, Ben Lansdell, Ross Brookwell, Ola Myklebost, Leonardo Meza-Zepeda, Andrew J Holloway, Florence Pedeutour, KH Andy Choo, Michael A Damore, Andrew J Deans, Anthony T Papenfuss, David M Thomas, None, The architecture and evolution of cancer neochromosomes. Cancer Cell. ,vol. 26, pp. 653- 667 ,(2014) , 10.1016/J.CCELL.2014.09.010
Mark G. Rush, Ravi Misra, Extrachromosomal DNA in eucaryotes Plasmid. ,vol. 14, pp. 177- 191 ,(1985) , 10.1016/0147-619X(85)90001-0
Hannelore Schmidt, Helge Taubert, Heidemarie Lange, Karen Kriese, Wolfgang Daniel Schmitt, Steve Hoffmann, Frank Bartel, Steffen Hauptmann, Small polydispersed circular DNA contains strains of mobile genetic elements and occurs more frequently in permanent cell lines of malignant tumors than in normal lymphocytes Oncology Reports. ,vol. 22, pp. 393- 400 ,(2009) , 10.3892/OR_00000450
S. Cohen, D. Segal, Extrachromosomal circular DNA in eukaryotes: possible involvement in the plasticity of tandem repeats Cytogenetic and Genome Research. ,vol. 124, pp. 327- 338 ,(2009) , 10.1159/000218136
Takahiro Kunisada, Hideo Yamagishi, Toyozo Sekiguchi, Intracellular location of small circular DNA complexes in mammalian cell lines. Plasmid. ,vol. 10, pp. 242- 250 ,(1983) , 10.1016/0147-619X(83)90038-0