The fslE homolog, FTL_0439 (fupA/B), mediates siderophore-dependent iron uptake in Francisella tularensis LVS.
作者： Bhaswati Sen , Alexis Meeker , Girija Ramakrishnan
DOI: 10.1128/IAI.00503-10
关键词: Virulence 、 Francisella tularensis 、 Francisella 、 Complementation 、 Tularemia 、 Siderophore 、 Biology 、 Microbiology 、 Bacterial vaccine 、 Mutant
摘要: The Gram-negative pathogen Francisella tularensis secretes a siderophore to obtain essential iron by TonB-independent mechanism. fslABCDE locus, encoding siderophore-related functions, is conserved among different strains. In the virulent strain Schu S4, fslE for utilization and growth under conditions of limitation. contrast, we found that deletion did not affect attenuated live vaccine (LVS). We one paralogs encoded in LVS genome, FTL_0439 (fupA/B), was able partially complement S4 ΔfslE mutant utilization. generated fupA/B background. ΔfupA/B showed reduced It secrete but unable utilize siderophore. Mutation both resulted defect greater severity. mutants replication J774.1A cells decreased virulence following intraperitoneal infection mice. Complementation mutation cis restored ability concomitantly virulence. Our results indicate plays significant role siderophore-mediated uptake mechanism whereas appears play secondary role. Variation acquisition mechanisms may contribute differences between
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