Absolute Quantification of the Model Biomarker Prostate-Specific Antigen in Serum by LC-MS/MS Using Protein Cleavage and Isotope Dilution Mass Spectrometry
作者： David R. Barnidge , Marcia K. Goodmanson , George G. Klee , David C. Muddiman
DOI: 10.1021/PR049963D
关键词: Isotope dilution 、 Dilution 、 Peptide 、 Mass spectrometry 、 Stable isotope ratio 、 Chromatography 、 Triple quadrupole mass spectrometer 、 Chemistry 、 Trypsin 、 Calibration curve
摘要: … an internal standard. Here, we investigate use of a standard LC−MS/MS platform for quantifying … 5,6 (where the term ‘protein' is a generic term typically referring to a polypeptide with an …
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