The Physicochemical Properties of Decellularized Extracellular Matrix-Coated 3D Printed Poly(ε-caprolactone) Nerve Conduits for Promoting Schwann Cells Proliferation and Differentiation

摘要: Although autologous nerve grafting remains the gold standard treatment for peripheral injuries, alternative methods such as development of guidance conduits have since emerged and evolved to counter many disadvantages grafting. However, efficacy viability current remain unclear in clinical trials. Here, we focused on a novel decellularized extracellular matrix (dECM) polydopamine (PDA)-coated 3D-printed poly(e-caprolactone) (PCL)-based conduits, whereby PDA surface modification acts an attachment platform further dECM attachment. We demonstrated that dECM/PDA-coated PCL possessed higher mechanical properties when compared human or animal nerves. Such modifications were proved affect cell behaviors. Cellular behaviors neuronal differentiation Schwann cells assessed determine efficacies conduits. There some cell-specific markers, Nestin, neuron-specific class III beta-tubulin (TUJ-1), microtubule-associated protein 2 (MAP2) analyzed by enzyme-linked immunosorbent assay, Nestin expressions found be 0.65-fold up-regulated, while TUJ1 2.3-fold up-regulated MAP2 2.5-fold Ctl. The methodology coating employed this study can used simple model immobilize onto results showed practical clinically viable tool promoting regenerative outcomes larger defects.