Development of an acute toxicity test with the tropical marine amphipod Parhyale hawaiensis.

摘要: There is a lack of suitable tropical marine species for ecotoxicity tests. An attractive model organism ecotoxicology the amphipod Parhyale hawaiensis, which already genetic and developmental studies. This widespread, can tolerate changes in salinity, easy to handle representative circumtropical regions. The aim this work was describe standardized procedures laboratory husbandry, define conditions acute toxicity tests, provide test results some reference toxicants. Culturing were established reconstituted seawater (30 ± 2 salinity), 24 ± 2 °C, photoperiod 12/12 h light/dark. Acute developed 96 h-exposure time, organisms at ages <7 days. miniaturized version test, based on 96-well microplates 200 µL exposure media provided consistent compared larger volumes (80-mL vials protocol). Ag, Cd, Cu, Zn ammonia determined P. hawaiensis previous other amphipods. We conclude that be successfully cultured effectively used testing. Further development use will enable reproducible investigations understudied vulnerable ecosystems.