A Metalloproteomics Study on the Association of Mercury With Breast Milk in Samples From Lactating Women in the Amazon Region of Brazil.
作者： Felipe André dos Santos , Bruna Cavecci , José Cavalcante Souza Vieira , Vanessa Pezza Franzini , Ademir Santos
DOI: 10.1007/S00244-015-0161-8
关键词: Chemistry 、 Electrospray ionization 、 Breast milk 、 Cold vapour atomic fluorescence spectroscopy 、 Environmental chemistry 、 Mercury (element) 、 Lysozyme 、 Chromatography 、 Mass spectrometry 、 Population 、 Tandem mass spectrometry
摘要: This study aimed to identify metalloproteins that lose their metal ions in the presence of mercury (Hg) and bind Hg breast milk samples collected from riverine population Madeira River, a tributary Amazon River. Initially, total was determined hair lactating women individuals who were contaminated followed by proteomic analysis through two-dimensional polyacrylamide gel electrophoresis after acetone precipitation. The obtained protein spots cold vapor atomic absorption spectrometry fluorescence spectrometry. These determinations indicated one spot, which then characterized electrospray ionization tandem mass Based on searches UniProt database, this spot identified as lysozyme C.
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Rejane Corrêa Marques, Avaliação da exposição ao mercúrio e seus compostos sobre o desenvolvimento neuropsicomotor em crianças de Porto Velho-RO Universidade Federal do Pará. ,(2002)
Elmer Cranton, Richard Passwater, Trace Elements Hair Analysis and Nutrition ,(1983)
P. Grandjean, E. Budtz-Jorgensen, R. F. White, P. J. Jorgensen, P. Weihe, F. Debes, N. Keding, Methylmercury Exposure Biomarkers as Indicators of Neurotoxicity in Children Aged 7 Years American Journal of Epidemiology. ,vol. 150, pp. 301- 305 ,(1999) , 10.1093/OXFORDJOURNALS.AJE.A010002
Felipe A Santos, Paula M Lima, Renato CF Neves, Paula M Moraes, Carlos A Pérez, Marcelo OA Silva, Marco AZ Arruda, Gustavo R Castro, Pedro de Magalhaes Padilha, None, Metallomic study on plasma samples from Nile tilapia using SR-XRF and GFAAS after separation by 2D PAGE: initial results Mikrochimica Acta. ,vol. 173, pp. 43- 49 ,(2011) , 10.1007/S00604-010-0522-Y
Tina Kold Jensen, Philippe Grandjean, Esben Budtz Jørgensen, Roberta F White, Frodi Debes, Pál Weihe, Effects of breast feeding on neuropsychological development in a community with methylmercury exposure from seafood Journal of Exposure Science and Environmental Epidemiology. ,vol. 15, pp. 423- 430 ,(2005) , 10.1038/SJ.JEA.7500420
Jerusa Simone Garcia, Cristiana Schmidt de Magalhães, Marco Aurélio Zezzi Arruda, Trends in metal-binding and metalloprotein analysis Talanta. ,vol. 69, pp. 1- 15 ,(2006) , 10.1016/J.TALANTA.2005.08.041
Paola Borella, Sergio Rovesti, Eva Caselgrandi, Annalisa Bargellini, Quality control in hair analysis: A systematic study on washing procedures for trace element determinations Mikrochimica Acta. ,vol. 123, pp. 271- 280 ,(1996) , 10.1007/BF01244400
C.P. Braga, A.C. Bittarello, C.C.F. Padilha, A.L. Leite, P.M. Moraes, M.A.R. Buzalaf, L.F. Zara, P.M. Padilha, Mercury fractionation in dourada (Brachyplatystoma rousseauxii) of the Madeira River in Brazil using metalloproteomic strategies. Talanta. ,vol. 132, pp. 239- 244 ,(2015) , 10.1016/J.TALANTA.2014.09.021
Andrej Shevchenko, Henrik Tomas, Jan Havli, Jesper V Olsen, Matthias Mann, None, In-gel digestion for mass spectrometric characterization of proteins and proteomes Nature Protocols. ,vol. 1, pp. 2856- 2860 ,(2006) , 10.1038/NPROT.2006.468
P.S Fadini, W.F Jardim, Is the Negro River Basin (Amazon) impacted by naturally occurring mercury Science of The Total Environment. ,vol. 275, pp. 71- 82 ,(2001) , 10.1016/S0048-9697(00)00855-X