DIFERENTES TÉCNICAS DE DIAGNÓSTICO EMPREGADAS NA ESTIMATIVA DE PREVALÊNCIA POPULACIONAL DE INFECÇÃO POR Cryptosporidium felis EM GATOS DOMICILIADOS NO MUNICÍPIO DE ARAÇATUBA, SÃO PAULO

摘要: The aim of this work was to compare the diagnostic methods by capture enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (nested-PCR) morphological analysis using technique flotation in water-ether followed modified Kinyoun staining (microscopy) estimate shedding rate Cryptosporidium spp. stool samples cats domiciled urban area municipality Aracatuba, Sao Paulo State. Zoonotic potential coccidian isolated feces investigated molecular characterization. A total 138 were collected random proportionally from cat population each seven census areas belonging area. There no disagreement between ELISA microscopy (p = 1.0000) or nested-PCR 0.1094); however, degree agreement varied substantial (Kappa 0.7948) moderate 0.4647), respectively, these methods. Specificity similar; showed lower sensitivity, justified association intensity optical density amplification 18S rRNA subunit. We detected 9.4% at least two With 95% confidence, we that oocysts ranged 4.5% 14.3% feline Aracatuba. All isolates sequenced 100% similarity with felis. conclude can contribute environmental contamination a municipality, although C. felis is not primary etiologic agent cryptosporidiosis humans.