Protein refolding method
作者： Ryosuke Yumioka , Daisuke Ejima
DOI:
关键词: Pulmonary surfactant 、 Arginine 、 A protein 、 Protein refolding 、 Chemistry 、 Chromatography 、 Aqueous solution 、 Derivative (chemistry) 、 Solubilization
摘要: The present invention provides a method for producing protein which has restored native higher-order structure by bringing lost its into contact at pH 6.5 to 9.0 with 1 3% aqueous solution of specific surfactant, such as lauroylglutamic acid obtain solubilized the protein; and then adding buffer containing arginine or an derivative concentration 0.1 1.2 M lower acid, in obtained mixture down 0.02 0.275%. According invention, it is possible easily restore while smoothly removing surfactant from protein.
lens.org参考文章(59)
Jacqueline A. Reynolds, [5] The role of micelles in protein—detergent interactions Methods in Enzymology. ,vol. 61, pp. 58- 62 ,(1979) , 10.1016/0076-6879(79)61007-8
Lisa D. Cabrita, Stephen P. Bottomley, Protein expression and refolding - a practical guide to getting the most out of inclusion bodies Biotechnol Annu Rev , 10 pp. 31-50. (2004). ,vol. 10, pp. 31- 50 ,(2004) , 10.1016/S1387-2656(04)10002-1
Ryosuke Yumioka, Daisuke Ejima, Tsutomu Arakawa, Method for improving recovery yield in protein purification with gel filtration chromatography ,(2006)
Richard R. Burgess, Refolding solubilized inclusion body proteins. Methods in Enzymology. ,vol. 463, pp. 259- 282 ,(2009) , 10.1016/S0076-6879(09)63017-2
S Tandon, P Horowitz, Detergent-assisted refolding of guanidinium chloride-denatured rhodanese. The effect of lauryl maltoside. Journal of Biological Chemistry. ,vol. 261, pp. 15615- 15618 ,(1986) , 10.1016/S0021-9258(18)66759-6
Timothy W. Evans, Mark W. Knuth, Purification of somatotropin from transformed microorganisms ,(1986)
Daisuke Ejima, Hajime Koyama, Tsutomu Arakawa, Method for Inactivating Viruses With Slightly Acidic Arginine ,(2008)
Richard R. Burgess, Purification of overproduced Escherichia coli RNA polymerase sigma factors by solubilizing inclusion bodies and refolding from Sarkosyl. Methods in Enzymology. ,vol. 273, pp. 145- 149 ,(1996) , 10.1016/S0076-6879(96)73014-8
Ryosuke Yumioka, Daisuke Ejima, Tsutomu Arakawa, Purification method which prevents denaturation of an antibody ,(2012)
Eliana De Bernardez Clark, Elisabeth Schwarz, Rainer Rudolph, Inhibition of aggregation side reactions during in vitro protein folding. Methods in Enzymology. ,vol. 309, pp. 217- 236 ,(1999) , 10.1016/S0076-6879(99)09017-5