作者: Joseph Heitman , Sharvari Gujja , Daniel John Lawson , Christopher A. Desjardins , Sean M. Sykes
DOI: 10.1101/132894
关键词: Chromosomal region 、 Phylogenetic tree 、 Population genomics 、 Cryptococcus neoformans 、 Genotype 、 Population 、 Biology 、 Molecular epidemiology 、 Genetics 、 Lineage (evolution)
摘要: Cryptococcus neoformans var. grubii is the causative agent of cryptococcal meningitis, a significant source mortality in immunocompromised individuals, typically HIV/AIDS patients from developing countries. Despite worldwide emergence this ubiquitous infection, little known about global molecular epidemiology fungal pathogen. Here we sequence genomes 188 diverse isolates and characterized major subdivisions, their relative diversity level genetic exchange between them. While most C. belong to one three lineages (VNI, VNII, VNB), some haploid show hybrid ancestry including that appear have recently interbred, based on detection large blocks each across chromosome. Many display evidence aneuploidy, which was detected for all chromosomes. In diploid (serotype A/A) hybrids with A/D) such aneuploidies resulted loss heterozygosity, where chromosomal region represented by genotype only parental isolate. Phylogenetic population genomic analyses Brazil revealed previously “African” VNB lineage occurs naturally South American environment. This suggests migration Africa America prior its diversification, supported finding ancestral recombination events different regions. The results provide substantial structure, showing multi-continental distributions demonstrating highly dispersive nature