Purification of DNA Oligos by denaturing polyacrylamide gel electrophoresis (PAGE).
作者： Sara Lopez-Gomollon , Francisco Esteban Nicolas
DOI: 10.1016/B978-0-12-418687-3.00006-9
关键词: DNA 、 Molecular-weight size marker 、 Chemistry 、 Chromatography 、 Chemical synthesis 、 Oligonucleotide Purification 、 Gel electrophoresis of nucleic acids 、 Polyacrylamide gel electrophoresis 、 Oligonucleotide 、 Electroelution
摘要: After chemical synthesis, the oligonucleotide preparation contains desired full-length but also all of DNA molecules that were aborted during each cycle in and by-products generated reactions. The purification oligonucleotides is a critical step for demanding applications where exact length or sequence important, longer than 50 bases. There are several methods increasing purity, choice which will depend on modifications their intended use. Polyacrylamide gel (PAGE purification) method when highest percentage desired. This chapter describes protocol using denaturing polyacrylamide electrophoresis, includes preparation, from slice by two different methods: diffusion electroelution. recommendations as well advice.
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