Effects of priming with cytokines on intracellular survival and replication of Rhodococcus equi in equine macrophages.

摘要: Abstract Rhodococcus equi is a common cause of pneumonia in foals and an opportunistic pathogen immunosuppressed people. The ability R. to survive replicate macrophages the basis its pathogenicity. Limited knowledge about role cytokines host defense against comes from studies mice intracellular survival equine unknown. objectives this study were determine effect priming with interferon (IFN)-γ, interleukin (IL)-1β, IL-4, IL-6, IL-10, or tumor necrosis factor (TNF)-α at various concentrations on virulent monocyte-derived (MDM), effects combinations same equi. MDM 10 adult horses primed recombinant doubling ranging 25 200 ng/mL prior infection Priming IFN-γ, TNF-α, IL-6 significantly decreased replication compared unprimed monolayers. In contrast, IL-10 IL-1β increased Pairwise listed above did not results synergism antagonism. This demonstrated that improved function whereas detrimental.