Accurate quantification of cells recovered by bronchoalveolar lavage.

摘要: Quantification of the differential cell count and total number cells recovered from lower respiratory tract by bronchoalveolar lavage is a valuable technique for evaluating alveolitis patients with inflammatory disorders tract. The most commonly used evaluation has been to concentrate centrifugation then determine using hemocytometer Wright-Giemsa-stained cytocentrifuge preparation. However, we have noted that percentage small present in original suspension greater than lymphocytes identified on preparations. Therefore, developed procedures determining counts collected Millipore filters stained hematoxylin-eosin (filter preparations) compared results performed filter preparations with...